https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Interferon-gamma inhibits seminal plasma induction of colony-stimulating factor 2 in mouse and human reproductive tract epithelial cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37555 TGFBR2 encoding TGFB receptor 2. Conversely, TGFB1 suppressed IFNG receptor 1 and 2 genes IFNGR1 and IFNGR2. These data identify IFNG as a potent inhibitor of the TGFB-mediated seminal fluid interaction with relevant reproductive tract epithelia in mice and human. These findings raise the prospect that IFNG in the male partner's seminal fluid impairs immune adaptation for pregnancy following coitus in women.]]> Thu 18 Feb 2021 09:50:20 AEDT ]]> Maternal smoke exposure impairs the long-term fertility of female offspring in a murine model https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29938 Thu 03 Feb 2022 12:18:20 AEDT ]]> Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1595 Sat 24 Mar 2018 08:30:41 AEDT ]]> The paradoxical relationship between stallion fertility and oxidative stress https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19260 Sat 24 Mar 2018 08:06:38 AEDT ]]> Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20640 Sat 24 Mar 2018 07:55:44 AEDT ]]> Transport of IgG across the blood-luminal barrier of the male reproductive tract of the rat and the effect of estradiol administration on reabsorption of fluid and IgG by the epididymal ducts https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:322 Sat 24 Mar 2018 07:42:42 AEDT ]]> Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41 Sat 24 Mar 2018 07:42:12 AEDT ]]> Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:59 Sat 24 Mar 2018 07:42:05 AEDT ]]> Formation and dissociation of sperm bundles in monotremes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27768 Sat 24 Mar 2018 07:40:48 AEDT ]]> Characterization of an L-amino acid oxidase in equine spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27633 L-tryptophan > L-tyrosine) were substrates for this enzyme, eliciting the dose- and time-dependent generation of ROS via mechanisms that were enhanced by cell death. This unexpected result was confirmed by analyses of ROS generation in subcellular sperm fractions, which again located a majority of LAAO activity to the sperm head. Equine cryopreservation medium was shown to contain sufficient quantities of aromatic amino acids to activate the LAAO system and generate ROS. The biological significance of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to suppress sperm motility but only if dead spermatozoa were present in the same suspension. The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. These results suggest the potential significance of LAAO activity in generating the oxidative stress associated with the cryopreservation of equine spermatozoa. It is possible that inhibitors of this enzyme system may facilitate the development of modified cryostorage regimes for clinical validation in vivo.]]> Sat 24 Mar 2018 07:34:05 AEDT ]]> Analysis of protein thiol changes occurring during rat sperm epididymal maturation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27256 Sat 24 Mar 2018 07:29:12 AEDT ]]> Defining the mechanisms by which the reactive oxygen species by-product, 4-hydroxynonenal, affects human sperm cell function https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27253 Sat 24 Mar 2018 07:29:09 AEDT ]]> Chlamydia muridarum infection-induced destruction of male germ cells and sertoli cells is partially prevented by Chlamydia major outer membrane protein-specific immune CD4 cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25153 Sat 24 Mar 2018 07:17:08 AEDT ]]> Unstable Foxp3+ regulatory T cells and altered dendritic cells are associated with lipopolysaccharide-induced fetal loss in pregnant Interleukin 10-deficient mice https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37675 −/−) mice exhibit altered local T cell responses in pregnancy, exhibiting pronounced hyperplasia in para-aortic lymph nodes draining the uterus with >6-fold increased CD4⁺ and CD8⁺ T cells compared with wild-type controls. Among these CD4⁺ cells, Foxp3⁺ T regulatory (Treg) cells were substantially enriched, with 11-fold higher numbers at Day 9.5 postcoitum. Lymph node hypertrophy in Il10−/− mice was associated with more activated phenotypes in dendritic cells and macrophages, with elevated expression of MHCII, scavenger receptor, and CD80. Affymetrix microarray revealed an altered transcriptional profile in Treg cells from pregnant Il10−/− mice, with elevated expression of Ctse (cathepsin E), Il1r1, Il12rb2, and Ifng. In vitro, Il10−/− Treg cells showed reduced steady-state Foxp3 expression, and polyclonal stimulation caused greater loss of Foxp3 and reduced capacity to suppress IL17 in CD4+Foxp3− T cells. We conclude that despite a substantially expanded Treg cell pool, the diminished stability of Treg cells, increased numbers of effector T cells, and altered phenotypes in dendritic cells and macrophages in pregnancy all potentially confer vulnerability to inflammation-induced fetal loss in Il10−/− mice. These findings suggest that IL10 has a pivotal role in facilitating robust immune protection of the fetus from inflammatory challenge and that IL10 deficiency could contribute to human gestational disorders in which altered T cell responses are implicated.]]> Fri 12 Mar 2021 10:01:59 AEDT ]]> TLR4 signaling is a major mediator of the female tract response to seminal fluid in mice https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37674 Fri 12 Mar 2021 10:01:58 AEDT ]]>